Lord Darnley, second husband of Mary, Queen of Scots, is found strangled following an explosion at the Kirk o' Field house in Edinburgh, Scotland, a suspected assassination.

Henry Stuart, Lord Darnley was King of Scotland as the second husband of Mary, Queen of Scots, from 29 July 1565 until his murder in 1567. Lord Darnley had one child with Mary, the future James VI of Scotland and I of England. Through his parents, he had claims to both the Scottish and English thrones. Less than a year after the birth of his son, Darnley was murdered at Kirk o' Field in 1567. Many contemporary narratives describing his life and death refer to him as simply Lord Darnley, his title as heir apparent to the Earldom of Lennox.

Mary, Queen of Scots, also known as Mary Stuart or Mary I of Scotland, was Queen of Scotland from 14 December 1542 until her forced abdication on 24 July 1567.

The Collegiate Church of St Mary in the Fields was a pre-Reformation collegiate church in Edinburgh, Scotland. Likely founded in the 13th century and secularised at the Reformation, the church's site is now covered by Old College.

Edinburgh is the capital city of Scotland and one of its 32 council areas. It is located in southeast Scotland and is bounded to the north by the Firth of Forth and to the south by the Pentland Hills. Edinburgh had a population of 506,520 in 2020, making it the second-most-populous city in Scotland and the seventh-most-populous in the United Kingdom. The wider metropolitan area had a population of 912,490 in the same year.