Isabella of Portugal (born 1503)

Isabella of Portugal was the empress consort of Charles V, Holy Roman Emperor, King of Spain, Archduke of Austria, and Duke of Burgundy. She was Queen of Spain and Germany, and Lady of the Netherlands from 10 March 1526 until her death in 1539, and became Holy Roman Empress and Queen of Italy in February 1530. She acted as regent of Spain during her husband's long absences.

May 1 is the 121st day of the year in the Gregorian calendar; 244 days remain until the end of the year.