Isabella of Portugal (died 1539)

Isabella of Portugal was the empress consort of Charles V, Holy Roman Emperor, King of Spain, Archduke of Austria, and Duke of Burgundy. She was Queen of Spain and Germany, and Lady of the Netherlands from 10 March 1526 until her death in 1539, and became Holy Roman Empress and Queen of Italy in February 1530. She acted as regent of Spain during her husband's long absences.

October 24 is the 297th day of the year in the Gregorian calendar; 68 days remain until the end of the year.