Charles Horace Mayo, American surgeon, co-founder of the Mayo Clinic (died 1939)

Charles Horace Mayo was an American medical practitioner and was one of the founders of the Mayo Clinic along with his brother William James Mayo, Augustus Stinchfield, Christopher Graham, Edward Star Judd Jr., Henry Stanley Plummer, Melvin Millet, and Donald Balfour.

Mayo Clinic is a private American academic medical center focused on integrated healthcare, education, and research. It maintains three major campuses in Rochester, Minnesota; Jacksonville, Florida; and Phoenix/Scottsdale, Arizona.